Bioethanol Production from Matured Leaves of Eichhornia Crassipes through Separate Hydrolysis and Fermentation (SHF): Basis for Instructional Laboratory Manual Development
DOI:
https://doi.org/10.65339/ijsair.V2.I2.529Keywords:
Bioethanol, Eichhornia Crassipes, Fermentation, Hydrolysis, Instructional Manual, Renewable Energy, Water HyacinthAbstract
This study investigated the production of bioethanol from matured leaves of water hyacinth (Eichhornia crassipes) using the Separate Hydrolysis and Fermentation (SHF) process and developed an instructional laboratory manual based on the experimental findings. Grounded in constructivist and experiential learning principles, the study employed an experimental research design under controlled laboratory conditions. Matured water hyacinth leaves collected from Balzain, Tuguegarao City, Cagayan were subjected to wet and dried pretreatment conditions, followed by acid pretreatment, enzymatic hydrolysis, fermentation using Saccharomyces cerevisiae, and ethanol quantification through Gas Chromatography (Shimadzu GC-2010). Results revealed that wet pretreated biomass produced a higher average bioethanol yield (3.870 g/L) than dried pretreated biomass (2.396 g/L). Statistical analysis indicated a significant difference between the two biomass conditions, confirming that moisture content influences ethanol production efficiency. The findings demonstrated the effectiveness of SHF in converting water hyacinth into bioethanol and highlighted the potential of this invasive aquatic plant as a renewable lignocellulosic feedstock. The study also resulted in the development of an experimental protocol procedure that serves as the basis for an instructional laboratory manual designed to support experiential, inquiry-based, and sustainability-oriented science learning. It is recommended that future studies optimize process parameters, explore alternative pretreatment methods and microbial strains, and evaluate pilot-scale applications of bioethanol production from water hyacinth. The study supports Sustainable Development Goal (SDG) 4 (Quality Education), SDG 7 (Affordable and Clean Energy), SDG 12 (Responsible Consumption and Production), and SDG 13 (Climate Action) through the integration of renewable energy research and contextualized science instruction. Its sustainability impact includes promoting environmental management through the utilization of invasive biomass, supporting renewable energy development, and strengthening sustainability-focused science education.
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