Diagnostic Performance of FilmArray and MALDI-TOF for the Detection of Klebsiella pneumoniae for Sepsis and Bloodstream Infections: A Meta-Analysis
DOI:
https://doi.org/10.65339/ijsair.V2.I2.475Keywords:
Bloodstream Infections, Intensive Care Unit, Klebsiella Pneumoniae, MALDI-TOF MS, Rapid Diagnostics, Sepsis, Conventional Blood CultureAbstract
This study evaluated the diagnostic performance of FilmArray and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in the rapid detection of Klebsiella pneumoniae among intensive care unit (ICU) patients with sepsis and bloodstream infections. The study aimed to determine the effectiveness of these rapid diagnostic methods compared with conventional blood culture in terms of diagnostic accuracy, turnaround time, and clinical applicability. A quantitative comparative research design was employed using clinical blood samples collected from ICU patients suspected of bloodstream infections. Standard laboratory procedures, including conventional blood culture, FilmArray, and MALDI-TOF MS analyses, were utilized to identify bacterial pathogens. Data were analyzed to compare the sensitivity, specificity, and speed of pathogen detection among the diagnostic approaches. Findings revealed that FilmArray and MALDI-TOF MS provided significantly faster identification of Klebsiella pneumoniae compared with conventional blood culture while maintaining high diagnostic accuracy. The rapid diagnostic systems enhanced timely clinical decision-making and supported earlier antimicrobial intervention in critically ill patients. However, conventional blood culture remained important as the standard confirmatory diagnostic method despite its longer processing time. The study concluded that integrating rapid molecular and proteomic diagnostic technologies in ICU settings can improve sepsis management and patient outcomes. It further recommended the adoption of FilmArray and MALDI-TOF MS alongside conventional blood culture to strengthen early detection and optimize antimicrobial therapy in healthcare institutions. The study supports Sustainable Development Goal (SDG) 3: Good Health and Well-Being by promoting improved diagnostic strategies for infectious diseases and enhanced patient care in critical healthcare settings. Its sustainability impact lies in strengthening healthcare efficiency, improving clinical outcomes, and supporting evidence-based infection management practices within healthcare institutions.
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